University of Zagreb School of Medicine

Participants will achieve basic knowledge about principles of mass spectrometry and other tools used in proteomic research. After completion of the course students will be able to distinguish between advantages and weaknesses of proteomic techniques and would be competent to know where to apply these techniques in particular studies.

Proteomics represents one of the key and fast-growing branches of molecular biology and medicine with capability to provide quantitative and qualitative analysis of proteins in cells, tissues and organism. Aim of this subject is general introduction to basic principles and methods of proteomics, specifically those based on mass spectrometry. Lectures will shortly describe history of proteomics and its basic strategies (protein electrophoresis, immunohistochemistry and Western-blot); mass spectrometry in global protein identification and quantification processes, as well as specific application in basic biomedical research. Participants will learn how to prepare the sample for analysis and will have opportunity to work with sophisticated proteomic machines (mass spectrometry, HPLC) and learn processing of results utilizing bioinformatics.

• Ang MY, Low TY, Lee PY, Wan Mohamad Nazarie WF, Guryev V, Jamal R. roteogenomics: From next-generation sequencing (NGS) and mass spectrometry-based proteomics to precision medicine. lin Chim Acta. 2019;498:38-46.
• Grgurevic L, Erjavec I, Grgurevic I, Dumic-Cule I, Brkljacic J, Verbanac D, Matijasic M, Paljetak HC, Novak R, Plecko M, Bubic-Spoljar J, Rogic D, Kufner V, Pauk M, Bordukalo-Niksic T, Vukicevic S. Systemic inhibition of BMP1-3 decreases progression of CCl4-induced liver fibrosis in rats. Growth Factors. 2017;35:201-215.
• Aebersold R, Mann M. 2003. Mass spectrometry-based proteomics. Nature. 422:198-207.
• Macek, B., Mann, M., Olsen, J.V. 2009. Global and site-specific quantitative phosphoproteomics: principles and applications. Ann Rev Pharmacol 49: 199-221
• Lipton, M.S., Pasa-Tolic, Lj. 2009. Mass Spectrometry of Proteins and Peptides. Springer, Berlin, ISBN: 9781934115480
• van Eyk, J.E., Dunn, J.M. (Editors). 2007. Clinical Proteomics: From Diagnosis to Therapy. Wiley-Vch, ISBN-13: 9783527316373
• Hotchkiss, J.L., Simpson, R.J. 2003. Proteins and Proteomics: A Laboratory Manual. Cold Spring Harbor Laboratory Press, ISBN:  9780879695545
• Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. 2002. Molecular Biology of the Cell. 4. Edition, Garland Science, ISBN: 0815340729

Lectures, seminars, practical work

Written exam